Introduction: training, training and specialization are inherent aspects of the nursing profession, considering the scientific and technological changes that occur at a dizzying pace in the health sciences. Objective: to evaluate the training program for nursing professionals on the appropriate collection of the neonatal metabolic screening sample. The study group consisted of 22 nursing professionals from health centers in the urban area of Tulcán. Methods: a survey validated by health experts was applied, where 3 criteria were evaluated: experience, updating and procedure. A rating scale was also created for each of the three criteria. Results: The need for nursing staff to update their knowledge about the Neonatal Metabolic Screening program implemented by the Ministry of Public Health is evident. Regarding the updating of knowledge, it is noted that professionals seem to receive and assimilate updates in a more homogeneous way, which could be the result of well-implemented continuing education programs or regular policies that promote professional updating. Discussion: it is suggested that while procedural training seems standardized, it may be insufficient, so it could benefit from more structured and more intensive approaches. Conclusions: a balance between personalization and standardization is crucial to optimize the quality and efficiency of the health services provided.