Listeria monocytogenes in artisanal fresh cheeses sold in public markets of the city of Cuenca, 2026
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Abstract
Introduction. Diseases associated with the consumption of contaminated food represent a significant public health concern. Among the bacterial agents of greatest importance is Listeria monocytogenes, which causes listeriosis, a severe disease that primarily affects pregnant women, newborns, older adults, and immunocompromised individuals. Artisanal fresh cheeses are susceptible to microbiological contamination due to variable production, handling, and storage conditions, particularly when the cold chain is not properly maintained. Objective. To determine the presence of Listeria monocytogenes in artisanal fresh cheeses sold at different points of sale in the city of Cuenca. Method. A total of 43 samples of artisanal fresh cheeses sold in different markets were collected and analyzed using microbiological detection methods based on the INEN-ISO 11290 standard. Compact Dry LM plates were used as a screening method for the presumptive identification of Listeria monocytogenes. Results: The presence of bacteria of the genus Listeria was detected in 79% of the samples analyzed, of which 18.6% corresponded to Listeria monocytogenes and 60.4% to Listeria spp. Conclusion. Microbiological contamination was detected in most of the artisanal fresh cheeses analyzed, representing a health risk to consumers and indicating deficiencies in the hygiene and marketing conditions of these products. General Area of Study: Biochemistry and Pharmacy. Specific area of study: Food Safety and Food Microbiology. Type of study: Original article.
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