Dental autotransplantation with i-PRF and apical sealing with Biodentine: a clinical case report with unfavorable evolution
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Abstract
Introduction: dental autotransplantation is a conservative therapeutic alternative indicated when implants are contraindicated. Objective: The objective of this report was to describe a clinical case of autotransplantation with application of injected fibrin-rich plasma (i-PRF) and apical sealing with biodentine. Methodology: This clinical case report was used to conduct a descriptive study. Results: A 32-year-old patient was treated in whom the upper left third molar (2.8) was transplanted to the site of the first upper left molar (2.6). Despite the use of i-PRF and the sealing of the dental apex with biodentine, the procedure presented clinical failure. Conclusions: This outcome was associated with persistent gingival inflammation secondary to poor oral hygiene, which favored bacterial colonization of the gingival sulcus and compromised early periodontal reattachment. In addition, the behavior of endodontic sealant cement in a humid environment may have contributed to bacterial microfiltration and the persistence of the inflammatory process. General area of study: Dentistry. Specific area of study: Endodontics. Type of article: Clinical case.
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Lazo Espinoza, A. G., Rodríguez Reyes, F. G., & Lugo Pinto, M. A. (2026). Dental autotransplantation with i-PRF and apical sealing with Biodentine: a clinical case report with unfavorable evolution. Anatomía Digital, 9(2), 6-20. https://doi.org/10.33262/anatomiadigital.v9i2.3638
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Articulos de revisión bibliográfica
References
1. Andreasen JO, Paulsen HU, Yu Z, Bayer T. A long-term study of 370 auto transplanted premolars. Part I. Surgical procedures and standardized techniques for monitoring healing. European Journal of Orthodontics [Internet]. 1990 [cited 2026 Jan.08];12(1):3–13. Available from: http://dx.doi.org/10.1093/ejo/12.1.3
2. Tsukiboshi M. Auto transplantation of teeth: requirements for predictable success. Dental Traumatology [Internet]. 2002 [cited 2026 Jan.08]; 18:157-180. Available from: http://dx.doi.org/10.1034/j.1600-9657.2002.00118.x
3. Czochrowska EM, Stenvik A, Bjercke B, Zachrisson BU. Outcome of tooth transplantation: survival and success rates 17–41 years posttreatment. American Journal of Orthodontics and Dentofacial Orthopedics [Internet]. 2002 [cited 2026 Jan 08]; 121(2):110-119; quiz 193. Available from: http://dx.doi.org/10.1067/mod.2002.119979
4. Shahbazian M, Jacobs R, Wyatt J, Denys D, Lambrichts I, Vinckier F, et al. Validation of the cone beam computed tomography-based stereolithographic surgical guide aiding auto transplantation of teeth: clinical case-control study. Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology [Internet]. 2013 [cited 2026 Jan.08];115(5):667–675. Available from: http://dx.doi.org/10.1016/j.oooo.2013.01.025
5. Verweij JP, Jongkees FA, Becking AG, Mensink G, Fiocco M, van Merkesteyn JPR. Auto transplantation of teeth using computer-aided rapid prototyping technology. International Journal of Oral and Maxillofacial Surgery [Internet]. 2017 [cited 2026 Jan.08];46(11):1466-1474. Available from: https://www.ijoms.com/article/S0901-5027(17)31417-0/abstract
6. Gao J, Guo L, Yang D. Application of computer-aided design combined with 3-dimensional-printed guide in tooth auto transplantation: a case report. BMC Oral Health [Internet]. 2026 [cited 2026 Jan.08];26:258. Available from: http://dx.doi.org/10.1186/s12903-025-07459-0
7. Dawood A, Marti Marti B, Sauret-Jackson V, Darwood A. 3D printing in dentistry. British Dental Journal [Internet]. 2015 [cited 2026 Jan.08];219:521-529. Available from: http://dx.doi.org/10.1038/sj.bdj.2015.914
8. Singh AK., Khanal N, Acharya N, Hasan MR, Saito T. What are the complications, success and survival rates for auto transplanted teeth? An overview of systematic reviews and metanalyses. Healthcare [Internet]. 2022 [cited 2026 Jan.08]; 10(5), 835. Available from: https://doi.org/10.3390/healthcare10050835
9. Jaber M, Prasad P, Akeil M, Moufti A, Al-Sammarraie A, Charaf Eddin C. Long-term evaluation of tooth transplantation: an umbrella review. Journal of Clinical Medicine [Internet]. 2024 [cited 2026 Jan.08]; 13(11), 3341. Available from: https://doi.org/10.3390/jcm13113341
10. Kharabe V, Thosar N, Fulzeleet P, Pramod Bane S, Gupta S. Comparative evaluation of setting time and sealing ability of two root end filling materials: an in vitro study. Journal Of Datta Meghe Institute of Medical Sciences University [Internet]. 2023 [cited 2026 Jan.08];18(3):468-474. Available from: https://doi.org/10.4103/jdmimsu.jdmimsu_104_21
11. Malkondu Ö, Kazandağ MK, Kazazoğlu E. A review on biodentine, a contemporary dentine replacement and repair material. BioMed Research International [Internet]. 2014 [cited 2026 Jan.08]; 2014(1):160951. Available from: https://onlinelibrary.wiley.com/doi/10.1155/2014/160951
12. Han L, Okiji T. Bioactivity evaluation of three calcium silicate-based endodontic materials. International Endodontic Journal [Internet]. 2013 [cited 2026 Jan.08]; 46(9):808-814. Available from: http://dx.doi.org/10.1111/iej.12062
13. Falkowska J, Chady T, Dura W, Droździk A, Tomasik M, Marek E, et al. The washout resistance of bioactive root-end filling materials. Materials [Internet]. 2023 [cited 2026 Jan.08]; 16(17):5757. Available from: www.mdpi.com/1996-1944/16/17/5757
14. Gollapudi M, Bajaj P, Oza RR. Injectable platelet-rich fibrin - a revolution in periodontal regeneration. Cureus [Internet]. 2022 [cited 2026 Jan.08]; 14(8):e28647. Available from: http://dx.doi.org/10.7759/cureus.28647
15. Hajibagheri P, Basirat M, Tabari-Khomeiran Z, Asadi-Aria A. The efficacy of platelet-rich fibrin (PRF) in post-extraction hard and soft tissue healing and associated complications: a systematic review and meta-analysis of split-mouth randomized clinical trials. BMC Oral Health [Internet]. 2025 [cited 2026 Jan.08]; 25(1):869. Available from: http://dx.doi.org/10.1186/s12903-025-06238-1
16. Alkofahi H, Maghaireh A, Fnaish M, Jarrah M, Bataineh M. Application of platelet-rich fibrin as regeneration assistant in immediate auto transplantation of third molar with unformed roots: case report and review of literature. Case Reports in Dentistry [Internet]. 2020 [cited 2026 Jan.08]; 2020(1):8170646. Available from: http://dx.doi.org/10.1155/2020/8170646
17. Alijani S, Ghamari A, Saleh AK, Salehzadeh M. Immediate auto transplantation of a mandibular premolar with complete roots to a fractured tooth site, using platelet-rich fibrin in an orthodontic patient, report of a case. Dental Research Journal (Isfahan) [Internet]. 2023 [cited 2026 Jan.08]; 20(1):34. Available from: http://dx.doi.org/10.4103/1735-3327.372651
18. SamavatiJame F, Alrmali A, Galindo-Fernández P, Stuhr S, Wang HL. Tooth auto transplantation outcomes using platelet-rich fibrin: a promising approach; case study. Clinical Advances in Periodontics [Internet]. 2025 [cited 2026 Jan.08]; 15(1):31-38. Available from: http://dx.doi.org/10.1002/cap.10287
19. Idris MI, Burhan AS, Hajeer MY, Sultan K, Nawaya FR. Efficacy of the injectable platelet-rich fibrin (i-PRF) in gingival phenotype modification: a systematic review and meta-analysis of randomized controlled trials. BMC Oral Health [Internet]. 2024 [cited 2026 Jan.08]; 24(1):1331. Available from: http://dx.doi.org/10.1186/s12903-024-05109-5
20. Silva FFV E, Chauca-Bajaña L, Caponio VCA, Cueva KAS, Velasquez-Ron B, Padín-Iruegas ME, et al. Regeneration of periodontal intrabony defects using platelet-rich fibrin (PRF): a systematic review and network meta-analysis. Odontology [Internet]. 2024 [cited 2026 Jan.08]; 112(4):1047-1068. Available from: http://dx.doi.org/10.1007/s10266-024-00949-7
21. Salinas Molina M, Cevallos Calero CM, Rodríguez Reyes FG. Dental auto transplantation with regenerative therapy using A-PRF (Advanced Platelet Rich Fibrin) and I PRF (Injectable Platelet Rich Fibrin). Anatomía Digital [Internet]. 2026Feb.27 [cited 2026Jan.12];9(1.2):105-21. Available from: https://cienciadigital.org/revistacienciadigital2/index.php/AnatomiaDigital/article/view/3612
22. Figueroa Ortiz BB, Rodríguez Reyes FG. Application of I-PRF in bone and periodontal healing after apicoectomy: clinical case. Anatomía Digital [Internet]. 2026Feb.25 [cited 2026Jan.12];9(1.2):83-104. Available from: https://cienciadigital.org/revistacienciadigital2/index.php/AnatomiaDigital/article/view/3607
23. Cherres Quiroz JA, Rodriguez Reyes FG. Intentional replantation with I-PRF for periapical healing. Anatomía Digital [Internet]. 2026Jan.5 [cited 2026Jan.12];9(1):82-5. Available from: https://cienciadigital.org/revistacienciadigital2/index.php/AnatomiaDigital/article/view/3577
24. González Guachizaca GV, Morocho Morocho NP, Lugo Pinto MA, Rodriguez Reyes FG. Endodontic regeneration with A-PRF and I-PRF in a permanent tooth with immature apex: a clinical case report. Anatomía Digital [Internet]. 2025Nov.13 [cited 2026Mar.12];8(4):57-3. Available from: https://www.cienciadigital.org/revistacienciadigital2/index.php/AnatomiaDigital/article/view/3558
25. Calle Coronel SP, Rodríguez Reyes FG, Piedra Andrade BR, Oviedo Delgado AN. Comparison of apical sealing capacity between bioceramic sealants and AH Plus with and without gutta-percha in endodontic treatments: an in vitro study. Anatomía Digital [Internet]. 2025Nov.13 [cited 2026Jan.12];8(4):74-94. Available from: https://cienciadigital.org/revistacienciadigital2/index.php/AnatomiaDigital/article/view/3559
26. Pulla Sarmiento GM, Rodríguez Reyes FG, Lugo Pinto MA. Guided endodontics. An alternative for high-complexity cases. a systematic narrative review. Conciencia Digital [Internet]. 2025Jun.25 [cited 2026Jan.12];8(2):65-8. Available from: https://www.cienciadigital.org/revistacienciadigital2/index.php/ConcienciaDigital/article/view/3420
2. Tsukiboshi M. Auto transplantation of teeth: requirements for predictable success. Dental Traumatology [Internet]. 2002 [cited 2026 Jan.08]; 18:157-180. Available from: http://dx.doi.org/10.1034/j.1600-9657.2002.00118.x
3. Czochrowska EM, Stenvik A, Bjercke B, Zachrisson BU. Outcome of tooth transplantation: survival and success rates 17–41 years posttreatment. American Journal of Orthodontics and Dentofacial Orthopedics [Internet]. 2002 [cited 2026 Jan 08]; 121(2):110-119; quiz 193. Available from: http://dx.doi.org/10.1067/mod.2002.119979
4. Shahbazian M, Jacobs R, Wyatt J, Denys D, Lambrichts I, Vinckier F, et al. Validation of the cone beam computed tomography-based stereolithographic surgical guide aiding auto transplantation of teeth: clinical case-control study. Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology [Internet]. 2013 [cited 2026 Jan.08];115(5):667–675. Available from: http://dx.doi.org/10.1016/j.oooo.2013.01.025
5. Verweij JP, Jongkees FA, Becking AG, Mensink G, Fiocco M, van Merkesteyn JPR. Auto transplantation of teeth using computer-aided rapid prototyping technology. International Journal of Oral and Maxillofacial Surgery [Internet]. 2017 [cited 2026 Jan.08];46(11):1466-1474. Available from: https://www.ijoms.com/article/S0901-5027(17)31417-0/abstract
6. Gao J, Guo L, Yang D. Application of computer-aided design combined with 3-dimensional-printed guide in tooth auto transplantation: a case report. BMC Oral Health [Internet]. 2026 [cited 2026 Jan.08];26:258. Available from: http://dx.doi.org/10.1186/s12903-025-07459-0
7. Dawood A, Marti Marti B, Sauret-Jackson V, Darwood A. 3D printing in dentistry. British Dental Journal [Internet]. 2015 [cited 2026 Jan.08];219:521-529. Available from: http://dx.doi.org/10.1038/sj.bdj.2015.914
8. Singh AK., Khanal N, Acharya N, Hasan MR, Saito T. What are the complications, success and survival rates for auto transplanted teeth? An overview of systematic reviews and metanalyses. Healthcare [Internet]. 2022 [cited 2026 Jan.08]; 10(5), 835. Available from: https://doi.org/10.3390/healthcare10050835
9. Jaber M, Prasad P, Akeil M, Moufti A, Al-Sammarraie A, Charaf Eddin C. Long-term evaluation of tooth transplantation: an umbrella review. Journal of Clinical Medicine [Internet]. 2024 [cited 2026 Jan.08]; 13(11), 3341. Available from: https://doi.org/10.3390/jcm13113341
10. Kharabe V, Thosar N, Fulzeleet P, Pramod Bane S, Gupta S. Comparative evaluation of setting time and sealing ability of two root end filling materials: an in vitro study. Journal Of Datta Meghe Institute of Medical Sciences University [Internet]. 2023 [cited 2026 Jan.08];18(3):468-474. Available from: https://doi.org/10.4103/jdmimsu.jdmimsu_104_21
11. Malkondu Ö, Kazandağ MK, Kazazoğlu E. A review on biodentine, a contemporary dentine replacement and repair material. BioMed Research International [Internet]. 2014 [cited 2026 Jan.08]; 2014(1):160951. Available from: https://onlinelibrary.wiley.com/doi/10.1155/2014/160951
12. Han L, Okiji T. Bioactivity evaluation of three calcium silicate-based endodontic materials. International Endodontic Journal [Internet]. 2013 [cited 2026 Jan.08]; 46(9):808-814. Available from: http://dx.doi.org/10.1111/iej.12062
13. Falkowska J, Chady T, Dura W, Droździk A, Tomasik M, Marek E, et al. The washout resistance of bioactive root-end filling materials. Materials [Internet]. 2023 [cited 2026 Jan.08]; 16(17):5757. Available from: www.mdpi.com/1996-1944/16/17/5757
14. Gollapudi M, Bajaj P, Oza RR. Injectable platelet-rich fibrin - a revolution in periodontal regeneration. Cureus [Internet]. 2022 [cited 2026 Jan.08]; 14(8):e28647. Available from: http://dx.doi.org/10.7759/cureus.28647
15. Hajibagheri P, Basirat M, Tabari-Khomeiran Z, Asadi-Aria A. The efficacy of platelet-rich fibrin (PRF) in post-extraction hard and soft tissue healing and associated complications: a systematic review and meta-analysis of split-mouth randomized clinical trials. BMC Oral Health [Internet]. 2025 [cited 2026 Jan.08]; 25(1):869. Available from: http://dx.doi.org/10.1186/s12903-025-06238-1
16. Alkofahi H, Maghaireh A, Fnaish M, Jarrah M, Bataineh M. Application of platelet-rich fibrin as regeneration assistant in immediate auto transplantation of third molar with unformed roots: case report and review of literature. Case Reports in Dentistry [Internet]. 2020 [cited 2026 Jan.08]; 2020(1):8170646. Available from: http://dx.doi.org/10.1155/2020/8170646
17. Alijani S, Ghamari A, Saleh AK, Salehzadeh M. Immediate auto transplantation of a mandibular premolar with complete roots to a fractured tooth site, using platelet-rich fibrin in an orthodontic patient, report of a case. Dental Research Journal (Isfahan) [Internet]. 2023 [cited 2026 Jan.08]; 20(1):34. Available from: http://dx.doi.org/10.4103/1735-3327.372651
18. SamavatiJame F, Alrmali A, Galindo-Fernández P, Stuhr S, Wang HL. Tooth auto transplantation outcomes using platelet-rich fibrin: a promising approach; case study. Clinical Advances in Periodontics [Internet]. 2025 [cited 2026 Jan.08]; 15(1):31-38. Available from: http://dx.doi.org/10.1002/cap.10287
19. Idris MI, Burhan AS, Hajeer MY, Sultan K, Nawaya FR. Efficacy of the injectable platelet-rich fibrin (i-PRF) in gingival phenotype modification: a systematic review and meta-analysis of randomized controlled trials. BMC Oral Health [Internet]. 2024 [cited 2026 Jan.08]; 24(1):1331. Available from: http://dx.doi.org/10.1186/s12903-024-05109-5
20. Silva FFV E, Chauca-Bajaña L, Caponio VCA, Cueva KAS, Velasquez-Ron B, Padín-Iruegas ME, et al. Regeneration of periodontal intrabony defects using platelet-rich fibrin (PRF): a systematic review and network meta-analysis. Odontology [Internet]. 2024 [cited 2026 Jan.08]; 112(4):1047-1068. Available from: http://dx.doi.org/10.1007/s10266-024-00949-7
21. Salinas Molina M, Cevallos Calero CM, Rodríguez Reyes FG. Dental auto transplantation with regenerative therapy using A-PRF (Advanced Platelet Rich Fibrin) and I PRF (Injectable Platelet Rich Fibrin). Anatomía Digital [Internet]. 2026Feb.27 [cited 2026Jan.12];9(1.2):105-21. Available from: https://cienciadigital.org/revistacienciadigital2/index.php/AnatomiaDigital/article/view/3612
22. Figueroa Ortiz BB, Rodríguez Reyes FG. Application of I-PRF in bone and periodontal healing after apicoectomy: clinical case. Anatomía Digital [Internet]. 2026Feb.25 [cited 2026Jan.12];9(1.2):83-104. Available from: https://cienciadigital.org/revistacienciadigital2/index.php/AnatomiaDigital/article/view/3607
23. Cherres Quiroz JA, Rodriguez Reyes FG. Intentional replantation with I-PRF for periapical healing. Anatomía Digital [Internet]. 2026Jan.5 [cited 2026Jan.12];9(1):82-5. Available from: https://cienciadigital.org/revistacienciadigital2/index.php/AnatomiaDigital/article/view/3577
24. González Guachizaca GV, Morocho Morocho NP, Lugo Pinto MA, Rodriguez Reyes FG. Endodontic regeneration with A-PRF and I-PRF in a permanent tooth with immature apex: a clinical case report. Anatomía Digital [Internet]. 2025Nov.13 [cited 2026Mar.12];8(4):57-3. Available from: https://www.cienciadigital.org/revistacienciadigital2/index.php/AnatomiaDigital/article/view/3558
25. Calle Coronel SP, Rodríguez Reyes FG, Piedra Andrade BR, Oviedo Delgado AN. Comparison of apical sealing capacity between bioceramic sealants and AH Plus with and without gutta-percha in endodontic treatments: an in vitro study. Anatomía Digital [Internet]. 2025Nov.13 [cited 2026Jan.12];8(4):74-94. Available from: https://cienciadigital.org/revistacienciadigital2/index.php/AnatomiaDigital/article/view/3559
26. Pulla Sarmiento GM, Rodríguez Reyes FG, Lugo Pinto MA. Guided endodontics. An alternative for high-complexity cases. a systematic narrative review. Conciencia Digital [Internet]. 2025Jun.25 [cited 2026Jan.12];8(2):65-8. Available from: https://www.cienciadigital.org/revistacienciadigital2/index.php/ConcienciaDigital/article/view/3420