Comparison of apical microleakage in teeth obturated with AH Plus®, Vioseal®, and BioRoot RCS®, with and without gutta-percha cone(s): evaluation by root sectioning and optical microscopy
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Abstract
Introduction: apical sealing is a determining factor for the success of endodontic treatment, as it prevents the leakage of fluids and microorganisms into the root canal system. Objective: The objective of this study was to compare apical microfiltration in filled teeth with the sealants AH Plus®, Vioseal® and BioRoot RCS®, with and without the use of gutta-percha cone(s), evaluation by root sections and light microscopy. Methodology: An in vitro experimental study was conducted using 30 human teeth extracted from uniradicular teeth, which were subjected to a standardized protocol of instrumentation and endodontic filling. The samples were randomly divided into six groups (n=5): AH Plus with gutta-percha, AH Plus without gutta-percha, Vioseal with gutta-percha, Vioseal without gutta-percha, BioRoot RCS with gutta-percha, and BioRoot RCS without gutta-percha. Subsequently, the teeth were subjected to a cutting process and evaluated by immersion in 2% methylene blue, recording the penetration of the dye at the apical level. Results: To determine whether the use of gutta-percha cone(s) influences apical microfiltration when different endodontic sealants are used. Conclusions: The apical sealing capacity was related to the intrinsic properties of the endodontic sealant. AH Plus® and Vioseal® showed less apical microfiltration, while BioRoot RCS® showed greater susceptibility to dye penetration and material resorption, with no evident influence of the use of gutta-percha cone(s). General area of study: Dentistry. Specific area of study: Endodontics. Type of article: In vitro experimental research.
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2. Vasavi N, Sirisha K. Micro-push-out bond strength of mineral-based root canal sealer in canals with different tapers. Journal of Oral Research [Internet]. 2021 [cited 2025 Dec.14];10(1):1–8. Available from: https://www.joralres.com/index.php/JOralRes/article/view/joralres.2021.015
3. Dsouza DP, Suvarna D, Shetty HS, Farhana D, Syed DA. To compare and evaluate the sealing ability of GuttaFlow Bioseal, BioRoot RCS and MTA Fillapex with AH Plus: an in vitro study. IOSR Journal of Dental and Medical Science [Internet]. 2020 [cited 2025 Dec.14];19(1):43–49. Available from: https://www.iosrjournals.org/iosr-jdms/papers/Vol19-issue8/Series-8/H1908084349.pdf
4. Endara Abbott ML. Estudio comparativo in vitro de la filtración apical con tres técnicas de obturación. Odontoestomatologia [Internet]. 2021 [citado 2025 Dic.14];23(38): e28. Disponible en: http://www.scielo.edu.uy/scielo.php?script=sci_arttext&pid=S1688-93392021000201208
5. Trope M, Bunes A, Debelian G. Root filling materials and techniques: bioceramics a new hope? Endodontic Topics [Internet]. 2015 [cited 2025 Dec.14];32(1):86–96. Available from: http://dx.doi.org/10.1111/etp.12074
6. Ørstavik D. Materials used for root canal obturation: technical, biological and clinical testing. Endod Topics [Internet]. 2005 [cited 2025 Dec.14];12(1):25–38. Available from: http://dx.doi.org/10.1111/j.1601-1546.2005.00197.x
7. Schäfer E, Zandbiglari T. Solubility of root-canal sealers in water and artificial saliva. International endodontic journal [Internet]. 2003 [cited 2025 Dec.14];36(10):660–669. Available from: http://dx.doi.org/10.1046/j.1365-2591.2003.00705.x
8. Camps J, Jeanneau C, El Ayachi I, Laurent P, About I. Bioactivity of a calcium silicate-based endodontic cement (BioRoot RCS): Interactions with human periodontal ligament cells in vitro. Journal of endodontics [Internet]. 2015 [cited 2025 Dec.14];41(9):1469–1473. Available from: http://dx.doi.org/10.1016/j.joen.2015.04.011
9. De-Deus G, Brandão MC, Leal F, Reis C, Souza EM, Luna AS, et al. Lack of correlation between sealer penetration into dentinal tubules and sealability in nonbonded root fillings: Tubular dentine sealer penetration and sealability. International Endodontic Journal [Internet]. 2012 [cited 2025 Dec.14];45(7):642–651. Available from: http://dx.doi.org/10.1111/j.1365-2591.2012.02023.x
10. Spident Co. Ltd. Vioseal Root Canal Sealer: instructions for use. Seoul, Korea [Internet]; 2019 [cited 2025 Dec.14]. Available from: https://www.spident.co.kr/en/sub/product/prod.php?mode=view&s_cate=12&idx=20
11. Zhou HM, Shen Y, Zheng W, Li L, Zheng YF, Haapasalo M. Physical properties of 5 root canal sealers. Journal of Endodontics [Internet]. 2013 [cited 2025 Dec.14];39(10):1281–1286. Available from: http://dx.doi.org/10.1016/j.joen.2013.06.012
12. Kontakiotis EG, Wu MK, Wesselink PR. Effect of sealer thickness on long-term sealing ability: a 2-year follow-up study. International Endodontic Journal [Internet]. 1997 [cited 2025 Dec.14];30(5):307–312. Available from: http://dx.doi.org/10.1046/j.1365-2591.1997.00087.x
13. Calle Coronel SP, Rodríguez Reyes FG, Piedra Andrade BR, Oviedo Delgado AN. Comparison of apical sealing capacity between bioceramic sealants and AH Plus with and without gutta-percha in endodontic treatments: an in vitro study. Anatomía Digital [Internet]. 2025Nov.13 [cited 2025Dec.14];8(4):74-. Available from: https://doi.org/10.33262/anatomiadigital.v8i4.3559