Prevalence of beta-lactamases in urine cultures of outpatients at the Vicente Corral Moscoso Hospital, period 2019 – 2022
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Abstract
Introduction: In recent years, a significant increase in the prevalence of resistant uropathies has been observed, with extended-spectrum beta-lactamase-producing bacteria being one of the most important groups, due to their mechanism of hydrolyzing beta-lactam antibiotics. Objective: To determine the prevalence of beta-lactamases in urine cultures of outpatients at the Vicente Corral Moscoso Hospital, period 2019 – 2022. Methodology: The methodology is quantitative research, with a cross-sectional, observational, descriptive-explanatory study. It was conducted at the Vicente Corral Moscoso Hospital, the study universe constitutes all the urine cultures of patients seen in the outpatient clinic in the period 2019 to 2022, with a total of 903 patients, the variables to be studied are: Age, sex, isolated microorganism, resistance to beta-lactam antibiotics, resistance to other antibiotics. The SPSS statistical package was used for data analysis. Results: the population was female with 80.3 %. Among the main bacteria producing extended-spectrum beta-lactamase in urine cultures from outpatients, Escherichia coli was identified as the most common bacterium in urine cultures with a total of 89.8%; however, only 21.0% reported positive for beta-lactamase, as well as 7.7% of Klebsiella oxytoca and 58.1% of Klebsiella pneumoniae. Conclusion: the prevalence and patterns of resistance and sensitivity of bacteria producing extended-spectrum beta-lactamases are comparable with both local and international epidemiology.
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Guamán Sánchez, M. F., Borja González, P. I., Gómez Muicela, E. I., & Rojas Lupercio, B. S. (2024). Prevalence of beta-lactamases in urine cultures of outpatients at the Vicente Corral Moscoso Hospital, period 2019 – 2022. Anatomía Digital, 7(4.1), 27-40. https://doi.org/10.33262/anatomiadigital.v7i4.1.3247
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Articulos de revisión bibliográfica
References
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2. Asamoah B, Labi A-K, Gupte HA, Davtyan H, Peprah GM, Adu-Gyan F, Nair D, Muradyan K, Jessani NS, Sekyere-Nyantakyi P. High resistance to antibiotics recommended in standard treatment guidelines in Ghana: a cross-sectional study of antimicrobial resistance patterns in patients with urinary tract infections between 2017–2021. International Journal of Environmental Research and Public Health [Internet]. 2022 [citado 4 Sept. 2024]; 19(24): 16556. Disponible en: www.mdpi.com/1660-4601/19/24/16556. https://doi.org/10.3390/ijerph192416556.
3. Labid A, Benouagueni S, Mehainaoui A, Kirane DG, Touati A. Antimicrobial resistance among gram-negative bacteria isolated in the newborn intensive care unit at abderrezak-bouhara Hospital of Skikda, Algeria. Microbial Drug Resistance [Internet]. 2023 [citado 4 Sept. 2024]; 29(6): 229–238. Disponible en: https://doi.org/10.1089/mdr.2022.0254.
4. Çi̇Çek A, Şemen V, Ejder NA, Gündoğdu D, Kalcan S, Köse F, Özgümüş O. Molecular epidemiological analysis of integron gene cassettes and TetA/TetB/TetD gene associations in Escherichia Coli Strains Producing Extended-Spectrum β-Lactamase (ESBL) in urine cultures. Advances in Clinical and Experimental Medicine [Internet]. 2021 [citado 4 Sept. 2024]; 31(1): 71–79. Disponible en: https://doi.org/10.17219/acem/142333.
5. Barnett BJ, Stephens DS. Urinary tract infection: an overview. The American Journal of the Medical Sciences [Internet]. 1997 [citado 4 Sept. 2024]; 314(4): 245–249. Disponible en: https://pubmed.ncbi.nlm.nih.gov/9332263/
6. Barrantes Jiménez K, Chacón Jiménez L, Arias Andrés M. El impacto de la resistencia a los antibióticos en el desarrollo sostenible. Población y Salud en Mesoamérica [Internet]. 2022 [cited 04 Sept 2024]; 19( 2 ): 305-329. Available from: https://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S1659-02012022000100305
7. Bedenić B, Meštrović T. Mechanisms of resistance in gram-negative urinary pathogens: from country-specific molecular insights to global clinical relevance. Diagnostics [Internet]. 2021 [citado 28 Aug. 2024]; 11(5): 800. Disponible en: https://doi.org/10.3390/diagnostics11050800.
8. Byron JK. Urinary tract infection. Veterinary Clinics of North America: Small Animal Practice [Internet]. 2019 [citado 4 Sept. 2024]; 49(2): 211–221. Disponible en: www.sciencedirect.com/science/article/abs/pii/S0195561618301396?via%3Dihub. https://doi.org/10.1016/j.cvsm.2018.11.005.
9. Agreda Orellana IS, Campoverde Rengifo J, Cabrera Gómez M, Maldonado Robles C, Arias Villalva R, Durazno Ortiz A, Aguilera Moreira V, Santamaría Loza E, Mosquera Meza L, Soria Álvarez C, Lema Ríos B, & Cárdenas Ruano M. Características microbiológicas de pacientes con urocultivos positivos del Hospital Universitario del Rio, Ecuador. Archivos Venezolanos de Farmacología y Terapéutica [Internet]. 2021 [citado 4 Sept. 2024]; 40(5): 506–514. Disponible en: www.redalyc.org/articulo.oa?id=55969711009.
10. Carvalho I, Carvalho JA, Martínez-Álvarez S, Sadi M, Capita R, Alonso-Calleja C, Rabbi F, Dapkevicius MdLNE, Igrejas G, Torres C, Poeta P. Characterization of ESBL-producing Escherichia coli and klebsiella pneumoniae isolated from Clinical Samples in a Northern Portuguese Hospital: predominance of CTX-M-15 and high genetic diversity. Microorganisms [Internet]. 2021 [citado 17 Sept. 2024]; 9(9): 1914. Disponible en: https://doi.org/10.3390/microorganisms9091914
11. Aitken SM, Clancy CJ. IDSA guidance on the treatment of antimicrobial resistant gram-negative infections. ContagionLive [Internet]. 2020 [citado 4 Sept. 2024]; 5(6): 1–5. Disponible en: www.contagionlive.com/view/idsa-guidance-on-the-treatment-of-antimicrobial-resistant-gram-negative-infections.
12. De Cueto M, Aliaga L, Alós J, Canut A, Los-Arcos I, Martínez JA, Mensa J, Pintado V, Rodriguez-Pardo D, Yuste JR, Pigrau C. Executive summary of the diagnosis and treatment of urinary tract infection: guidelines of the Spanish Society of Clinical Microbiology and Infectious Diseases (SEIMC). Enfermedades Infecciosas y Microbiología Clínica [Internet]. 2016 [citado 4 Sept. 2024]; 35(5): 314–320. Disponible en: https://doi.org/10.1016/j.eimc.2016.11.005
13. Panamá Illescas TA, Gallegos Merchan JD. Resistencia antimicrobiana en Escherichia coli aislada de urocultivos. Revista Vive [Internet]. 2021 [citado 4 Sept. 2024]; 4(12): 521–533. Disponible en: https://doi.org/10.33996/revistavive.v4i12.110.
14. Jijón Jiménez SM, Santos Imbago KL. Prevalencia de enterobacterias productoras de Betalactamasas de Espectro Extendido (BLEE) en pacientes ambulatorios en un laboratorio clínico de Quito [Tesis de pregrado, Universidad de las Américas UDLA, Quito, Ecuador ] [Internet]. 2020 [citado 4 Sept. 2024]. Disponible en: http://dspace.udla.edu.ec/handle/33000/13381
15. Perozo Mena AJ, Castellano González MJ. Detección de betalactamasas de espectro extendido en cepas de la familia Enterobacteriaceae. Kasmera [Internet]. 2009 [citado 4 Sept. 2024]; 37(1): 25–37. Disponible en: http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0075-52222009000100004&lng=es.
16. Solís MB, Romo S, Granja M, Sarasti JJ, Paz y Miño A, Zurita J. Infección comunitaria del tracto urinario por Escherichia coli en la era de resistencia antibiótica en Ecuador. MetroCiencia [Internet]. 2022 [citado 19 Sept. 2024];30(1):37-48. Disponible en: https://revistametrociencia.com.ec/index.php/revista/article/view/321
17. Castañeda Torres KE, Díaz Velásquez S. Prevalencia de cepas de escherichia coli productoras de betalactamasas de espectro extendido y de carbapenemasas aisladas de pacientes ambulatorios con itu en laboratorios clínicos privados de la ciudad de Chiclayo, mayo - diciembre del 2018 [Tesis de pregrado, Universidad Nacional Pedro Ruiz Gallo, Lambayeque, Perú] [Internet]. 2020 [citado 4 Sept. 2024]. Disponible en: https://repositorio.unprg.edu.pe/handle/20.500.12893/9374
18. Miranda J, Pinto J, Faustino M, Sánchez-Jacinto B, Ramírez F. Resistencia antimicrobiana de uropatógenos en adultos mayores de una clínica privada de Lima, Perú. Revista Peruana de Medicina Experimental y Salud Pública [Internet]. 2019 [citado 8 junio 2024]; 36(1): 87-92. Disponible en: https://doi.org/10.17843/rpmesp.2019.361.3765.