Gut microbiota in dogs with acute haemorrhagic gastroenteritis
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Published:
Nov 1, 2022
Keywords:
Fecal Microbiota; coprocultive; dysbiosis; coproparasitic.
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Abstract
Introduction. The intestinal microbiota (IM) is composed of a set of bacteria, which produce metabolites that influence the health of the host. Alterations in diversity and composition are known as dysbiosis. Objective. To identify the intestinal microbiota in canines with acute hemorrhagic gastroenteritis. Methodology. The feces of 10 canines were analyzed using standard bacteriological cultures and their qualitative assessment by direct coproparasitic. Results. 69.4% of the isolated bacteria are part of the normal intestinal microbiota, mostly bacteria of the Enterobacteriaceae family, and in 30.6% bacteria considered pathogenic were isolated (Shigella 23% and Aeromona hydrophila 7.6%). In 100% of the samples in the direct evaluation an increase in the intestinal microbiota was reported. Conclusion. Bacteriological cultures did not allow to identify all the bacteria present in the feces, while direct evaluation did allow to identify alterations in the amount of bacteria, determining dysbiosis states in less time. Molecular diagnosis would allow the identification of all bacteria present in both healthy patients and patients with gastroenteric diseases.
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Gárate Machuca, C. A., & Armas Ariza, J. C. (2022). Gut microbiota in dogs with acute haemorrhagic gastroenteritis. ConcienciaDigital, 5(4.2), 45-56. https://doi.org/10.33262/concienciadigital.v5i4.2.2380
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References
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Pilla, R. y Suchodolski, J. (2021). The Gut Microbiome of Dogs and Cats, and the Influence of Diet. Vet. Clinic. North Ame.: Small Anim. Pract. 51(3):605-621. https://doi.org/10.1016/j.cvsm.2021.01.002.
Suchodolski, J. (2016). Diagnosis and interpretation of intestinal dysbiosis in dogs and cats. Vet. J. 2(15):30-37. https://doi.org/10.1016/j.tvjl.2016.04.011.
Benvenuti, E., Bottero, E., Pierini, A., Gori, E. y Marchetti, V. (2020). The swab-sampled dry fecal cytology in healthy dogs and in dogs with acute and chronic diarrhea: a pilot study. Japanese J. Vet. Res. 68(3):51-158. https://doi.org/10.14943/jjvr.68.3.151
Blake, A., Guard, B., Honneffer, J., Lidbury, J., Steiner, J. y Suchodolski, J. (2019). Altered microbiota, fecal lactate, and fecal bile acids in dogs with gastrointestinal disease. Plos One. 14(10). https://doi.org/10.1371/journal.pone.0224454
Chandler, S., Cunningham, M., Lund, M.; Khanna, C., Naramore; Patel, A. y Day, M. (2017). Obesity and Associated Comorbidities in People and Companion Animals: A One Health Perspective. Journal of Comparative Pathology. 156(4):296-309. https://www.sciencedirect.com/science/article/pii/S0021997517301226
Handl, S., German, A., Holden, S., Dowd, S., Steiner, J. y Heilmann, R. (2013). Faecal microbiota in lean and obese dogs. Microbiology Ecology. 84(2):332-43. https://doi.org/10.1111/1574-6941.12067
Thomson, P., Santibáñez, R., Rodríguez-Salas, C., Flores-Yañez, C. y Garrido, D. (2022). Differences in the composition and predicted functions of the intestinal microbiome of obese and normal weight adult dogs. Peer J. 10. https://doi.org/10.7717/peerj.12695
Unterer, S., Busch, K. (2021). Acute Hemorrhagic Diarrhea Syndrome in Dogs. Vet. Clinic. North Ame.: Small Anim. Pract. 51(1):79-92. https://doi.org/10.1016/j.cvsm.2020.09.007
Honaker, R., Shmalberg, J., Tanprasertsuk, J., Perry, L., Massey, D. y JHA, A. (20209. Characterization of gut microbiomes of household pets in the United States using a direct-to-consumer approach. Plos One. 15(2). https://doi.org/10.1371/journal.pone.0227289.
Honneffer, J., Steiner, J., Lidbury, J. Y Suchodolski, J. (2019). Variation of the microbiota and metabolome along the canine gastrointestinal tract. Metabolomics. 13(26):1-20. https://doi.org/10.1007/s11306-11017-11165-11303.
Honneffer, J. y Minamoto, Y. (2014). Microbiota alterations in acute and chronic gastrointestinal. World J. Gastroent. 20(44):16489-97. https://doi.org/10.3748/wjg.v20.i44.16489.
Huang, Z., Pan, Z., Yang, R., Bi, Y. y Xiong, R. (2019). The canine gastrointestinal microbiota: early studies and research frontiers. Gut Microbes. 11(4):635-654. https://doi.org/10.1080/19490976.2019.1704142.
Kieler, I., Shamzir- Kamal, S., Vitger, A., Nielsen, D., Lauridsen, C. y Bjornvad, C. (2017). Gut microbiota composition may relate to weight loss rate in obese pet dogs. Vet. Med. Sci. 3(4):252-262. https://doi.org/10.1002/vms3.80.
Kim, J., An, J., Kim, W., Lee, S. yCho, S. (2017). Differences in the gut microbiota of dogs (Canis lupus familiaris) fed a natural diet or a commercial feed revealed by the Illumina MiSeq platform. Gut Pathog. 9(68). https://doi.org/10.1186/s13099-017-0218-5.
Suchodolski, J. (2021). Analysis of the gut microbiome in dogs and cats. Vet. Clin. Pathol. 50(1):6-17. https://doi.org/10.1111/vcp.13031.
Li, Q., Larouche - Lebel, E., Loughran, K.; Huh, T. y Suchodolski, J. (2021). Gut dysbiosis and its associations with gut microbiota-derived metabolites in dogs with myxomatous mitral valve disease. Msystems. 6(2). https://doi.org/10.1128/mSystems.00111-21.
Ramadhani, M., Indarjulianto, S., Nururrozi, A. y Raharjo, S. (2021). Case Report: Diagnosis and Treatment of Enteritis Caused by Bacterial in a Dog. BIO Web of Conferences. 33(6). https://doi.org/10.1051/bioconf/20213306003.
Werner, E., Suchodolski, J.S., Lidbury, J.A., Steiner, J.M. y Hartmann, K. (2020). Unterer, S. Diagnostic value of fecal cultures in dogs with chronic diarrhea. J. Vet. Intern. Med. 35(1):199-208. https://doi.org/10.1111/jvim.15982.
Pilla, R. y Suchodolski, J. (2021). The Gut Microbiome of Dogs and Cats, and the Influence of Diet. Vet. Clinic. North Ame.: Small Anim. Pract. 51(3):605-621. https://doi.org/10.1016/j.cvsm.2021.01.002.
Suchodolski, J. (2016). Diagnosis and interpretation of intestinal dysbiosis in dogs and cats. Vet. J. 2(15):30-37. https://doi.org/10.1016/j.tvjl.2016.04.011.