Activity Evaluation of three proteolytic enzymes as dairy biocatalysts

Abstract

The study of proteolytic enzymes at an industrial level is of great importance since their catalytic utility may be used especially in the dairy industry, so the objective of the study was to obtain three proteolytic enzymes from pineapple (Annanas-cosmosus L), papaya (Carica-papaya) and fig (Ficus-carica L), for which different latex extraction techniques were used, applying a completely random design and Tukey as a statistical test, with 5 repetitions and one size of an experimental unit of 5kg, resulting in highly significant differences between the three enzymes studied, while the methods of mechanical breakdown for pineapple, longitudinal cuts for papaya and fig were used to obtain the latex. Physicochemical evaluations of 7.62 Brix and 5.48 pH are obtained for papaya, 12.12 Brix and 2.20 pH for pineapple and 7.24 Brix with 6.48 pH for fig, while that, in what corresponds to the yield, it can be mentioned that with 18 g / Kg ficin is the enzyme with the best result. For the enzymatic activity, a completely randomized design with a unit size of 0.0375mg of enzyme was used, using milk as a substrate for the catalysis process, reporting values ​​of .15.74 U / mg protein with a time of 73.92sec for bromelain, 15.09 U / mg protein with a clotting time of 61.44sec for papain and 14.87 U / mg protein with a time of 58 seconds for ficin, indicating that the results show highly significant differences and demonstrating that Ficin is the enzyme that has an effective and efficient enzyme speed and clotting time.