Antimicrobial resistance of Staphylococcus aureus isolated from artisanal cheeses in the northern market of Cuenca, Ecuador
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Abstract
Introduction. Staphylococcus aureus is a microorganism capable of causing foodborne illnesses and may be present in artisanal products, such as cheese, whose contamination can be linked to deficiencies in hygienic conditions during production and handling. Objective. To identify the presence and evaluate the antimicrobial resistance of Staphylococcus aureus isolated from artisanal cheese marketed at the Northern Market of Cuenca, Ecuador. Methodology. A quantitative, descriptive, and cross-sectional study was conducted, in which 30 samples of artisanal cheese were analyzed. Results. S. aureus was identified in 36.7% of the samples, with counts exceeding the limit established by regulations. Furthermore, 45.5% of the isolates showed resistance to penicillin, while the other antimicrobials evaluated showed susceptibility. Conclusion. The presence of S. aureus and antimicrobial resistance were detected in the artisanal cheese analyzed, which could be associated with deficiencies in hygienic and sanitary conditions and factors related to the selection and dissemination of resistant microorganisms. General area of study: Biochemistry and Pharmacy. Specific area of study: Food microbiology. Type of study: Original articles.
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2. Taylor TA, Tobin EH, Unakal CG. Staphylococcus aureus Infection. StatPearls Publishing, Treasure Island [Internet]. 2025 [cited 2026 May 10]. Available from: http://www.ncbi.nlm.nih.gov/books/NBK441868/
3. Taddese D, Abdurahaman M, Debelo M, Shumi E, Urgessa G, Kefyalew D, et al. Antimicrobial resistance characterization of Staphylococcus aureus from different animal food origins in Jimma, South Western Ethiopia. Discover Food [Internet]. 2025 [cited 2026 May 10];5(1):27. Available from: https://doi.org/10.1007/s44187-025-00300-1
4. Liang T, Liang Z, Wu S, Ding Y, Wu Q, Gu B. Global prevalence of Staphylococcus aureus in food products and its relationship with the occurrence and development of diabetes mellitus. Medicine Advances [Internet]. 2023 [cited 2026 May 10];1(1):53-78. Available from: https://doi.org/10.1002/med4.6
5. Cieza MYR, Bonsaglia ECR, Rall VLM, dos Santos MV, Silva NCC. Staphylococcal Enterotoxins: description and importance in food. Pathogens [Internet]. 2024 [cited 2026 June 21];13(8):676. Available from: https://doi.org/10.3390/pathogens13080676
6. Léguillier V, Pinamonti D, Chung-Ming C, Gunjan, Mukherjee R, Himanshu, et al. A review and meta-analysis of Staphylococcus aureus prevalence in foods. The Microbe [Internet]. 2024 [cited 2026 March 2]; 4(100131):100131. Available from: https://doi.org/10.1016/j.microb.2024.100131
7. Organización Mundial de la Salud [OMS] [Internet]. Promoting safe food handling. 2025 [cited 2026 March 10]. Available from: https://www.who.int/activities/promoting-safe-food-handling
8. Agencia Nacional de Regulación, Control y Vigilancia Sanitaria [ARCSA] [Internet]. Las BPM garantizan la inocuidad en la cadena de producción de los alimentos procesados. 2026 [citado 2 de marzo de 2026]. Disponible en: https://www.controlsanitario.gob.ec/las-bpm-garantizan-la-inocuidad-en-la-cadena-de-produccion-de-los-alimentos-procesados/
9. González-Ochoa L, Agudo-Peralta MG, Vélez-Zamora L, Baculima-Suárez J, Flores-García M. Escherichia coli y coliformes totales en superficies inertes del patio de comidas del terminal terrestre Cuenca, Ecuador. FACSALUD-UNEMI [Internet]. 2023 [citado 2 marzo 2026];7(13):127-133. Disponible en: https://ojs.unemi.edu.ec/index.php/facsalud-unemi/article/view/1910
10. Gajewska J, Chajęcka-Wierzchowska W, Zadernowska A. Occurrence and characteristics of staphylococcus aureus strains along the production chain of raw milk cheeses in Poland. Molecules [Internet]. 2022 [cited 2026 March 10];27(19):6569. Available from: https://doi.org/10.3390/molecules27196569
11. Organización Mundial de la Salud [OMS] [Internet]. Resistencia a los antimicrobianos. 2021 [citado 10 de mayo de 2026]. Disponible en: https://www.who.int/es/news-room/fact-sheets/detail/antimicrobial-resistance
12. Organización Panamericana de la Salud [OPS] [Internet]. Resistencia a los antimicrobianos. 2024 [citado 10 de mayo de 2026]. Disponible en: https://www.paho.org/es/temas/resistencia-antimicrobianos
13. Organización Panamericana de la Salud [OPS] [Internet]. Resistencia Antimicrobiana en Producción Animal. 2025 [citado 2 de marzo de 2026]. Disponible en: https://www.paho.org/es/panaftosa/resistencia-antimicrobiana-produccion-animal
14. Feyissa N, Alemu T, Jirata Birri D, Dessalegn A. Isolation, identification, and determination of antibiogram characteristics of Staphylococcus aureus in cow milk and milk products (yoghurt and cheese) in West Showa Zone, Ethiopia. International Dairy Journal [Internet]. 2023 [cited 2026 May 10];137:105503. Available from: https://doi.org/10.1016/j.idairyj.2022.105503
15. Kayili E, Sanlibaba P. Prevalence, characterization and antibiotic resistance of Staphylococcus aureus isolated from traditional cheeses in Turkey. International Journal of Food Properties [Internet]. 2020 [cited 2026 May 10];23(1):1441-1451. Available from: https://doi.org/10.1080/10942912.2020.1814323
16. Castro RD, Pedroso SHSP, Sandes SHC, Silva GO, Luiz KCM, Dias RS, et al. Virulence factors and antimicrobial resistance of Staphylococcus aureus isolated from the production process of Minas artisanal cheese from the region of Campo das Vertentes, Brazil. Journal of Dairy Science [Internet]. 2020 [cited 2026 July 12];103(3):2098-2110. Available from: https://doi.org/10.3168/jds.2019-17138
17. Escobar S, Albuja A, Tene K, Jara H, Ramírez J. Análisis microbiológico y resistencia a antimicrobianos del queso fresco que se expende en un mercado, de la ciudad de Riobamba. Perfiles [Internet]. 2023 [citado 10 de mayo de 2026];1(30):13-23. Disponible en: https://doi.org/10.47187/perf.v1i30.223
18. Feijoó Armijos SP, Pinos Sarmiento ES, Ortiz Tejedor JG. Identificación de Staphylococcus aureus a partir de queso fresco expendido en mercados y centros comerciales de la ciudad de Cuenca. Anatomía Digital [Internet]. 2023 [citado 19 de abril de 2026];6(3.3):103-118. Disponible en: https://doi.org/10.33262/anatomiadigital.v6i3.3.2708
19. Instituto Ecuatoriano de Normalización [INEN] [Internet]. NTE INEN-ISO 707:2014. Leche y productos lácteos. Directrices para la toma de muestras. 2014 [citado 2 de marzo 2026]. Disponible en: https://pdfcoffee.com/nte-inen-iso-707-ext-2-pdf-free.html
20. CompactDryTM, Manual del usuario final [Internet]. 2021 [citado 2 de septiembre de 2026]. Disponible en: https://es.scribd.com/document/655415192/CompactDryTM-End-User-Manual-Version2-spanish
21. Instituto Ecuatoriano de Normalización [INEN] [Internet]. Norma técnica ecuatoriana - NTE INEN 1528:2012. 2012 [citado 3 de marzo 2026]. Disponible en: https://es.scribd.com/document/208472907/Norma-Inen-1528-Queso-Fresco
22. Klempt M, Franz CMAP, Hammer P. Characterization of coagulase-negative staphylococci and macrococci isolated from cheese in Germany. Journal of Dairy Science [Internet]. 2022 [cited 2026 March 2];105(10):7951-7958. Available from: https://doi.org/10.3168/jds.2022-21941
23. Roldan Tacuri GN, Wazhima Zhunio MC, Torres Segarra SM. Determinación de Staphylococcus aureus en quesos frescos del mercado 9 de octubre de la ciudad de Cuenca, Agosto 2023. Tesla Revista Científica [Internet]. 2024 [citado 21 de junio de 2026];4(1): e295. Disponible en: https://doi.org/10.55204/trc.v4i1.e295
24. Villa Cárdenas KP, Peralta Rodríguez KI, Torres Segarra SM. Identificación de Staphylococcus aureus en quesos expendidos en el mercado el Arenal Cuenca- Ecuador en el período marzo 2023. Anatomía Digital [Internet]. 2023 [citado 12 de julio de 2026]; 6(3.1):6–18. Disponible en: https://doi.org/10.33262/anatomiadigital.v6i3.1.2628
25. Farfán Sari KF, Romero Zhagui GR, Ortiz Tejedor JG. Susceptibilidad antimicrobiana y enterotoxinas en Staphylococcus aureus aislado de queso fresco expendido en mercados municipales de la ciudad de Cuenca – Ecuador. Anatomía Digital [Internet]. 2023 [citado 10 de mayo de 2026]; 6(4):22–40. Disponible en: https://doi.org/10.33262/anatomiadigital.v6i4.2704
26. Vásquez Requejo JJ, Quiroz Zárate FA, Sánchez Mego DA, Pesantes Corcuera PE, Julca Alarcón KA, Rivera Salazar CA. Susceptibilidad antibiótica de Staphylococcus aureus procedentes de quesos artesanales expendidos en mercados de Jaén. Revista Científica Dékamu Agropec [Internet]. 2025 [citado 1 de mayo de 2026];6(1):1-6. Disponible en: https://doi.org/10.55996/dekamuagropec.v6i1.259
27. El Mammery A, Ramírez de Arellano E, Cañada-García JE, Cercenado E, Villar-Gómara L, Casquero-García V, et al. An increase in erythromycin resistance in methicillin-susceptible Staphylococcus aureus from blood correlates with the use of macrolide/lincosamide/streptogramin antibiotics. EARS-Net Spain (2004–2020). Frontiers in Microbiology [Internet]. 2023 [cited 2026 June 21]; 14:1220286. Available: https://doi.org/10.3389/fmicb.2023.1220286