Dental autotransplantation with i-PRF and apical sealing with Biodentine: a clinical case report with unfavorable evolution

Mon, 06 Apr 2026 17:42:48 +0000

Introduction: dental autotransplantation is a conservative therapeutic alternative indicated when implants are contraindicated. Objective: The objective of this report was to describe a clinical case of autotransplantation with application of injected fibrin-rich plasma (i-PRF) and apical sealing with biodentine. Methodology: This clinical case report was used to conduct a descriptive study. Results: A 32-year-old patient was treated in whom the upper left third molar (2.8) was transplanted to the site of the first upper left molar (2.6). Despite the use of i-PRF and the sealing of the dental apex with biodentine, the procedure presented clinical failure. Conclusions: This outcome was associated with persistent gingival inflammation secondary to poor oral hygiene, which favored bacterial colonization of the gingival sulcus and compromised early periodontal reattachment. In addition, the behavior of endodontic sealant cement in a humid environment may have contributed to bacterial microfiltration and the persistence of the inflammatory process. General area of study: Dentistry. Specific area of study: Endodontics. Type of article: Clinical case.

Comparison of apical microleakage in teeth obturated with AH Plus®, Vioseal®, and BioRoot RCS®, with and without gutta-percha cone(s): evaluation by root sectioning and optical microscopy

Mon, 06 Apr 2026 17:47:56 +0000

Introduction: apical sealing is a determining factor for the success of endodontic treatment, as it prevents the leakage of fluids and microorganisms into the root canal system. Objective: The objective of this study was to compare apical microfiltration in filled teeth with the sealants AH Plus®, Vioseal® and BioRoot RCS®, with and without the use of gutta-percha cone(s), evaluation by root sections and light microscopy. Methodology: An in vitro experimental study was conducted using 30 human teeth extracted from uniradicular teeth, which were subjected to a standardized protocol of instrumentation and endodontic filling. The samples were randomly divided into six groups (n=5): AH Plus with gutta-percha, AH Plus without gutta-percha, Vioseal with gutta-percha, Vioseal without gutta-percha, BioRoot RCS with gutta-percha, and BioRoot RCS without gutta-percha. Subsequently, the teeth were subjected to a cutting process and evaluated by immersion in 2% methylene blue, recording the penetration of the dye at the apical level. Results: To determine whether the use of gutta-percha cone(s) influences apical microfiltration when different endodontic sealants are used. Conclusions: The apical sealing capacity was related to the intrinsic properties of the endodontic sealant. AH Plus® and Vioseal® showed less apical microfiltration, while BioRoot RCS® showed greater susceptibility to dye penetration and material resorption, with no evident influence of the use of gutta-percha cone(s). General area of study: Dentistry. Specific area of study: Endodontics. Type of article: In vitro experimental research.

Anatomía Digital

Dental autotransplantation with i-PRF and apical sealing with Biodentine: a clinical case report with unfavorable evolution

Comparison of apical microleakage in teeth obturated with AH Plus®, Vioseal®, and BioRoot RCS®, with and without gutta-percha cone(s): evaluation by root sectioning and optical microscopy