Investigación:
microorganismos en materias primas de establecimientos vs. capacitaci&oacut=
e;n
a manipuladores en una universidad pública
Research: microorganisms in raw materials from
establishments vs. training for handlers in a public university
&=
nbsp;
1
Brenda Regina
López Cárcamo &nbs=
p;
&nbs=
p; https://orcid.o=
rg/0000-0001-8621-9457
Doctorando en Cien=
cias
de la Investigación- Universidad Mariano Gálvez, Escuela
Estudios de Posgrado. Profesora del curso de Control Microbiológic=
o de
Alimentos de Experiencias Docentes con la Comunidad.
Jefa del Laborator=
io
de Control Microbiológico de Alimentos de la Universidad
Pública de Guatemala.
brendaregina7@profesor.usac.edu.gt ;
=
Artículo
de Investigación Científica y Tecnológica
Enviado: 10/08/2022
Revisado: 25/09/2022
Aceptado: 24/10/2022
Publicado: 14/11/2022
DOI: https://doi.org/10.33262/anatomiadigita=
l.v5i4.1.2392
&=
nbsp;
Cítese:
López
Cárcamo, B. R. (2022). Investigación: microorganismos en
materias primas de establecimientos vs. capacitación a manipulador=
es
en una universidad pública . Anatom&iac=
ute;a
Digital, 5(4.1), 84-102. https://doi=
.org/10.33262/anatomiadigital.v5i4.1.2392
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oner
sus resultados investigativos y científicos en la misma medida que=
se
promueva mediante su intervención cambios positivos en la
sociedad. https:=
//anatomiadigital.org
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"Times New Roman"'>
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ves
4.0 International. Copia de la licencia: http://creativecommons.org/licenses/by-n=
c-nd/4.0/
Palabras claves:
Resumen
Intr=
oducción:
Las Buenas Prácticas de
manufactura-BPM- tienen relevancia al momento de seleccionar las materias
primas para la preparación de alimentos; además considerando
que el que manipula los alimentos debe estar muy bien capacitado para
seleccionar dichas materias primas y con ello evitar la contaminaci&oacut=
e;n
alimentaria; por ello este artículo permitirá indicar si en=
los
establecimientos de una Universidad Pública logran evitar la
contaminación alimentaria. Objetivos: El objetivo de este
artículo es demostrar y contribuir que las muestras de alimentos
obtenidas en establecimientos de alimentos cumplen con inocuidad alimenta=
ria
y por otro lado se pretende comparar e interpretar que, si hay microorgan=
ismos
frecuentes, estos pueden ser aislados de los alimentos obtenidos de
establecimientos de alimentos, pero que con capacitación en buenas
prácticas de manufactura logren mejorar la selección de
materias primas. Metodología: Se consideró 455 resultados obtenidos en el
laboratorio de control microbiológico de alimentos, de la Universi=
dad
Pública de Guatemala y se les realizó el análisis fr=
ecuente
de indicador de contaminación fecal, la cual es la Escherichia =
coli ;
posteriormente se realizó un segundo vigilancia de microorganismos
patógenos presentes en los alimentos, a los cuales a más de=
un
alimento se le realizó dos o más patógenos, seg&uacu=
te;n
el alimento procesado y con ello compararlo a los valores de aceptabilidad
del Reglamento Centroamericano-RTCA-. Por último, se realizaron 18
capacitaciones de -BPM-; por último, para su análisis se
aplicó el programa de Excel para la Interpretación de resul=
tados.
Resultados: De las 455 muestras analizadas tuvieron Escherichia coli como indicador =
de
contaminación fecal; por lo que se llevó a cabo un segundo
muestreo para determinar microorganismos patógenos indicando que
fueron 215 alimentos y se realizó de un mismo alimento, entre 1 y 3
patógenos más frecuentes que se concentran en dicha materia
prima; para lo cual fue un total de 600 análisis
microbiológicos; de dichos resultados se presentaron dentro de un rango f=
uera
del parámetro de aceptabilidad, el 14.12 % (12) presentó Gr=
upo
Coliforme, 98.82 % (84) E. coli , 96.49 % (110) Mohos y levaduras y=
75
% (78) Recuento Aeróbico Total. Conclusiones: S e debe
implementar en los establecimientos Buenas Prácticas de Manufactura
-BPM-, así como mínimo capacitar dos veces al año a =
los
manipuladores; con el fin de lograr incrementar la higiene e inocuidad
alimentaria y con ello mantener los límites de inocuidad alimentar=
ia
en dichos establecimientos.
&Aac=
ute;rea
de estudio: (oncología, gastroenterología, etc.)
Keywords:
Foodborne Illness, Training, pathogens, bacter=
ia,
viruses, parasites. =
Abstract Good Manufacturing Practices -BPM- are relevant=
when selecting raw materials for food preparation; also considering that t=
he one who handles food must be very well trained to select said raw materi=
als and thereby avoid food contamination; For this reason, this article wil=
l indicate whether the establishments of a Public University manage to avoi=
d food contamination. Objectives: The objective of this article is t=
o demonstrate and contribute that the food samples obtained in food establi=
shments comply with food safety and on the other hand it is intended to com=
pare and interpret that, if there are frequent microorganisms, these can be=
isolated from the food obtained from food establishments, but with trainin=
g in good manufacturing practices they can improve the selection of raw mat=
erials. Methodology: 455 results obtained in the microbiological foo=
d control laboratory of the Public University of Guatemala were considered =
and frequent analysis of the fecal contamination indicator, which is Escher=
ichia coli, was performed; Subsequently, a second surveillance of pathogeni=
c microorganisms present in the food was carried out, to which two or more =
pathogens were carried out on more than one food, depending on the processe=
d food, and with this, it was compared to the acceptability values &=
#8203;of the Central American Regulation-RTCA-. Finally, 18 -BPM- trainings=
were conducted; Finally, for its analysis, the Excel program for the Inter=
pretation of results was applied. Results: Of the 455 samples analyz=
ed, they had Escherichia coli as an indicator of fecal contamination; There=
fore, a second sampling was carried out to determine pathogenic microorgani=
sms, indicating that there were 215 foods and it was made from the same foo=
d, between 1 and 3 more frequent pathogens that are concentrated in said ra=
w material; for which it was a total of 600 microbiological analyses; of th=
ese results were presented within a range outside the acceptability paramet=
er, 14.12% (12) presented Coliform Group, 98.82% (84) E. coli, 96.49% (110)=
Molds and yeasts and 75% (78) Aerobic Count Total. Conclusions: Goo=
d Manufacturing Practices -BPM- should be implemented in establishments, as=
well as training handlers at least twice a year; to achieve increased hygi=
ene and food safety and thereby maintain food safety limits in said establi=
shments. Study area: (oncology, gastroenterology, etc.) &nbs=
p;
&=
nbsp;
Introducción
Para
el departamento de Epidemiología las enfermedades transmitidas por
alimentos- ETA’s- constituyen un importante problema de salud a nivel
mundial, estas enfermedades se producen por el consumo de agua o alimentos
contaminados con microorganismos, parásitos o bien las sustancias
tóxicas que ellos producen (1) .
La
propagación y el alcance de las ETA’s está llegando a s=
er un
problema global, en donde las costumbres, hábitos alimentarios de los
consumidores y el factor tiempo hacen que ya no consuman en su hogar; porque
las diversas variedades de productos que se encuentran en el mercado hacen =
que
se incremente su comercialización y por ende se incrementan los peli=
gros
a la salud humana; que pueden ir desde el transporte de un lugar a otro, co=
mo
de un país a otro y esto puede repercutir e incidir en brotes
alimentarios (2) .
Las
ETA’s pueden ser intoxicaciones o infecciones, siendo las Infecciones=
las
que son transmitidas por alimentos y que esta enfermedad resulta de la
ingestión de alimentos que contienen microorganismos, tales como vir=
us,
bacterias, parásitos, que son perjudiciales y que se encuentran vivo=
s;
tales como Salmonella sp p, el v=
irus
de la Hepatitis A y Triquinella spirallis , entre otros (3) .
Así
también tenemos la intoxicación causada por alimentos, la cua=
l es
una enfermedad que resulta de la ingestión de toxinas o venenos que
están presentes en el alimento ingerido, que han sido producidas por
hongos o bacterias, aunque estos microorganismos ya no estén present=
es
en el alimento; entre ellos tenemos a la toxina botulínica y la
enterotoxina de Staphylococcus spp (2) .
Los
síntomas más comunes de las ETA’s son vómitos,
dolores abdominales, diarrea y fiebre, también pueden presentarse
síntomas neurológicos, ojos hinchados, dificultades renales,
visión doble, etc. Estos síntomas pueden variar dependiendo d=
e la
cantidad de bacterias presentes o de toxinas presentes en el alimento; de la
cantidad de alimento consumido y del estado de salud de la persona, entre o=
tros
factores (4) .
Para
las personas sanas, la mayoría de las ETA’s son enfermedades
pasajeras, que sólo duran un par de días y sin ningún =
tipo
de complicación, pero para las personas más susceptibles como=
son
los niños, los ancianos, las mujeres embarazadas o los que se encuen=
tran
enfermos pueden ser más severas y dejar secuelas o incluso hasta
provocar la muerte (5)
Por
lo mencionado existen las causas que según indican que la
etiología de estas enfermedades es diversa y que entre las causas qu=
e influyen
pueden ser la adición de aditivos alimentarios, metales tóxic=
os,
agentes químicos, toxinas marinas, así como las de origen
biológico; siendo dentro de estas últimas las producidas por
virus, bacterias, hongos y parásitos entre otras (6) .
Se
menciona además que las enfermedades virales transmitidas por los
alimentos y el agua son mucho menos conocidas que las demás y estas
pueden ser por astro virus, norovirus, rotavirus, adenovirus, enterovirus,
virus de la hepatitis entre otros (6) .
Dentro
de las causas microbiológicas están la Salmonella spp , Listeria
monocytogenes, Aeromonas hidrop=
hila ,
Plesiomonas shigelloide , Yersinia enterocolítica,
Campylobacter jejuni, Escherichia coli , infecciones por vibrios y Clostridium spp, Shigella spp, Bacillus
cereus, Staphylococcus aureus entre otros (7) .
Respecto
a las enfermedades transmitidas por parásitos son producidas por Giardia lambia, Entamoeba histolytica,
Crytosporidium parvum , entre otras (8) .
En
base a lo anterior se presentan los microorganismos más frecuentes,
donde se presenta el origen, transmisión y síntomas frecuente=
s en
humanos; siendo:
El microorganismo Campylobacter jejuni es encontrada en el tracto intestinal de l=
os
animales y aves, leche cruda, aguas sin tratar, aguas residuales y lodo;
así como su transmisión es por el agua, leche cruda, carnes
crudas o sin cocinar, aves y mariscos contaminados y sus síntomas son
fiebre, dolor de cabeza y dolor muscular seguida con diarreas (algunas veces
con sangre) dolor abdominal y nauseas pueden aparecer de 2 a 5 días
después de haber ingerido el alimento, puede durar de 7 a 10 d&iacut=
e;as
(9) .
La Listeria monocytogenes es encontrada en el tracto intestinal de
algunos mamíferos, leche cruda, aguas sin tratar, su transmisi&oacut=
e;n
es por aguas contaminadas, leche cruda, carne de res cruda sin cocinar, jug=
o de
manzanas o cidra de manzanas sin pasteurizar, frutas y vegetales sin cocina=
r y
de persona a persona; los síntomas son diarreas o diarreas con sangr=
e,
calambres abdominales, náuseas, y malestar; pueden comenzar de 2 a 5
días después de haber ingerido el alimento, durando como 8
días, Algunas personas en especial los más jóvenes, las
personas de edad avanzada, pueden desarrollar el síndrome
hemolítico urémico (HUS, por sus siglas en inglés) que
puede causar daño a los riñones (10) .
Salmonella la cual
incluye sobre más de 2300 tipos es encontrada en el tracto intestina=
l y
las heces fecales de animales, pero la&nbs=
p;
Salmonella Enteritidis se le ha aislado en huevos, huevos
crudos y sin cocinar, aves y carnes; leche cruda y productos lácteos;
pescados y mariscos y personas que manejan alimentos; entre los sínt=
omas
que se presentan son Stomach Dolor de estómago, diarreas, nausea,
escalofríos, fiebre, y dolor de cabeza que usualmente aparece de 8 a=
72
horas después de haber ingerido el alimento y puede durar de 1 a 2
días (11) .
Clostridium Botulinum es ampliamente distribuido en la
naturaleza, suelo, agua y en las plantas, y en el tracto intestinal de los
animales y pescados y mariscos; crece solamente en presencia de poco o de
ningún oxígeno, además es un microorganismo que produce una toxina que causa la
enfermedad; además se le encuentra en alimentos mal enlatados, ajo en
aceite, alimentos empacados al vacío y herméticamente; se ind=
ica
que presenta una toxina que afecta el sistema nervioso; los síntomas
usualmente aparecen de 18 a 36 horas, pero puede aparecer algunas veces en
sólo 4 horas o hasta 8 días después de ingerir el
alimento, se observa visión doble, parpados caídos, problemas=
al
hablar y al tragar, y dificultad al respirar. Puede ser fatal de 3 a 10
días si no es tratada.
Clostridium
perfringens es
encontrada en el suelo, polvo, aguas residuales, tracto intestinal de anima=
les
y humanos, crece solamente con presencia de poco o de ningún
oxígeno; es conocido como el “germen de cafetería”=
; ya
que numerosos brotes de enfermedades han resultado por alimentos dejados por
largos períodos en mesas de vapor o a temperatura ambiental; es un
microorganismo que puede ser destruida al cocinar, pero algunas esporas
productoras de esporas pueden sobrevivir. Dentro los síntomas que se
presentan son las diarreas y dolores causados por gases que podrían
aparecer de 8 a 24 horas después de haber ingerido el alimento;
usualmente dura como 1 día, pero síntomas más severos
pueden persistir de 1 a 2 semanas (4) .
Escherichia
coli
O157:H7 es un microorganismo frecuentemente encontrado en el tracto intesti=
nal
de algunos mamíferos, leche cruda, aguas sin tratar; una de las cepa=
s de
E. coli que puede causar enfermedades en los humanos; además aparece=
en
aguas contaminadas, leche cruda, carne de res cruda sin cocinar, jugo de
manzanas o cidra de manzanas sin pasteurizar, frutas y vegetales sin cocina=
r; y
de persona a persona. Dentro de los síntomas que presenta son las
diarreas o diarreas con sangre, calambres abdominales, náuseas, y
malestar; pueden comenzar de 2 a 5 días después de haber inge=
rido
el alimento, durando como 8 días. Algunas personas en especial los
más jóvenes, las personas de edad avanzada pueden desarrollar=
el
síndrome hemolítico urémico (HUS, por sus siglas en
inglés) que puede causar daño a los riñones (12) .
Shigella (sobre
más de30 tipos) se la encuentra en el tracto intestinal humano;
raramente encontrado en otros animales; además de persona a persona
vía ruta fecal-oral, así como es la contaminación feca=
l de
los alimentos y agua. Muchos de los brotes de enfermedades han resultado de
alimentos, especialmente ensaladas, preparadas por personas con higiene
personal pobre. Se ha indicado que este microorganismo forma parte de las
enfermedades referidas como “shigellosis” o disentería
bacilar; además presente diarreas con sangre, y mucosidad, fiebre,
calambres estomacales, escalofríos y vómitos y que de 12 a 50
horas después de la ingestión de la bacteria; puede durar por
unos pocos días hasta 2 semanas (13) .
Staphylococcus aureus se la
encuentra e n
humanos (piel, cortaduras infectadas, granos, nariz, y en la garganta),
además la transmisión es de persona a persona a través=
de
los alimentos mal manejados; así como se multiplica rápidamen=
te a
temperatura ambiental produciendo una toxina que causa la enfermedad
(intoxicación) y dentro de los síntomas que presenta son la
náusea severa, calambres abdominales, vómitos y diarreas, ocu=
rren
de 1 a 6 horas después de haber ingerido el alimento,
recuperación dentro de 2 a 3 días y toma más tiempo si
ocurre deshidratación severa (14)
Dentro del riesgo
personal en consumo de alimentos puede ser de alto, m=
ediano
o bajo riesgo para las personas, según edad, vulnerabilidad, entre o=
tras
y que se requiere de expertos con experiencia para determinarlo; por lo que=
se
deben contemplar varios aspectos, como: identificación del peligro d=
onde
se describa el microorganismo encontrado y la descripción del alimen=
to;
caracterización del peligro dentro del cual se describen los efectos
adversos a la salud y que son causados por el microorganismo; así co=
mo
información disponible en la literatura de la dosis-respuesta que se=
ha tenido
en el país u otros similares en humanos; además se requiere d=
e la
evaluación de la exposición o datos de prevalencia del peligr=
o en
la cadena alimentaria del país de origen de la contaminación<=
span
style=3D'mso-spacerun:yes'> de datos de consumo del mismo; se
requiere además de la caracterización del riesgo o
información del número de casos y efectos adversos resultante=
s de
la exposición al microorganismo, relacionadas con el alimento o
categorización del riesgo: esto último basado en dos criterio=
s:
en severidad y prevalencia, en información
del manejo del riesgo o descripción del sector industrial y los
controles relevantes; así como por último la Informació=
;n
relacionada con las opciones del manejo del riesgo=
(15) .
Asimismo, es de menci=
onar
que para cumplir con todo lo mencionado anteriormente se requiere aplicar l=
as
buenas prácticas de manufactura- BPM - ,
las cuales, para Montero originario de Ecuador, indica que las Buenas
Prácticas de Manufactura son una herramienta básica para la
elaboración de productos seguros para el consumo humano, que tiene c=
omo
base la higiene e inocuidad alimentaria (16) .
Arteta,
indica que las BPM son los requisitos generales mínimos que se deben
considerar en cuanto a instalaciones y procesos que debe establecer una emp=
resa
como procedimiento básico en la elaboración de un producto
inocuo. Además, se indica que el manejo higiénico y saneamien=
to
de los alimentos implican diversas etapas necesarias durante el proceso de
elaboración de estos; en las cuales se aplican las buenas
prácticas de manipulación en los alimentos. =
Se entiende entonces que es
fundamentalmente necesario en una empresa de alimentos tener y aplicar las =
BPM,
como rutina para ser aplicada permanentemente. Actualmente en el mundo una =
gran
cantidad de países los que han adoptado con carácter obligato=
rio
desde hace décadas este sistema y las encargadas velan por el
cumplimiento de estos y son las instituciones gubernamentales las que ejerc=
en
el control de alimentos (17) .
Para la aplicaci&oacu=
te;n
de buenas prácticas en Guatemala se tiene que =
p ara (18) , las BPM son principios básicos y
prácticas generales de higiene en la manipulación,
preparación, elaboración, envasado, almacenamiento, transport=
e y
distribución de los alimentos para el consumo humano; por lo que en =
los
diversos establecimientos se debe aplicar la correcta implementación=
de
las BPM y esto hace que se puede llevar un control de las Enfermedades
Transmitidas por Alimentos (ETA’s).
Es por ello por lo que se estima que hasta un 2=
0%
de las ETA’s son causadas por malas prácticas de higiene de los
trabajadores, y hasta un 14% se debe a la contaminación cruzada, don=
de
no realizan la separación de alimentos crudos con alimentos cocinado=
s.
Se puede indicar que en el capítulo V del
Código de Salud de la República de Guatemala expresa que es
responsabilidad de los propietarios de los establecimientos y expendedores =
de
alimentos, que se cumplan todas las normas sanitarias que aseguren la inocu=
idad
de estos. Todos los establecimientos deben contar con una licencia sanitaria
extendida por el Ministerio de Salud. Además, indica que el personal=
no
debe padecer enfermedades y que debe cumplir también con todas las
normas sanitarias que aseguren la seguridad higiénica de todos los
consumidores (19) .
Por otro l ado, la persona encar=
gada
de la manipulación de alimentos juega un papel importante en la
contaminación de éstos, por lo que el manipulador es responsa=
ble
de seguir las normas de higiene.
La
Organización de las Naciones Unidas para la Agricultura y la Aliment=
ación
define que la seguridad alimentaria es cuando toda persona tiene un acceso
físico y económico en todo momento a tener suficiente alimento
inocuo y nutritivo para la satisfacción de las necesidades alimentar=
ias,
estableciéndose para ello cuatro condiciones para cumplir con esta
seguridad alimentaria; siendo entre ellas, la disponibilidad y oferta de
alimentos adecuados; presencia de estabilidad de oferta sin fluctuaciones ni
escasez en el acceso a los alimentos, así como poseer la capacidad de
adquirirlos y mantener la buena calidad e inocuidad de los mismos.
Por
lo tanto, los controles de los alimentos se deben de realizar desde la
producción primaria, donde deben realizarse de manera que el alimento
sea inocuo y apto para el uso que se destina; se debe realizar un monitoreo
para evitar el uso de zonas donde el medio ambiente represente una amenaza =
para
la inocuidad de los alimentos, controlar los posibles contaminantes, las pl=
agas
y las enfermedades de animales y/o plantas, de manera que no representen una
amenaza para la inocuidad de éstos (20) .
Por
lo mencionado se deben adoptar prácticas y medidas que permitan aseg=
urar
la producción de alimentos en condiciones de higiene apropiadas. Todo
esto para reducir la probabilidad de que se origine un peligro que pueda
menoscabar la inocuidad de alimentos o su aptitud para el consumo en etapas
posteriores de la cadena alimentaria (21) .
Metodolo=
gía
Para este artículo se
consideró los 455 resultados obtenidos en el laboratorio
microbiológico de alimentos, de la Universidad Pública de
Guatemala, que se les realizó vigilancia de microorganismos
patógenos presentes en los alimentos, a los cuales a más de un
alimento se le realizó dos o más patógenos, segú=
;n
el alimentos procesado y donde la muestra son los 182 establecimientos de
alimentos que obtuvieron resultados microbiológicos con valores >=
3
NMP/g o ml y/o >10 UFC/g o ml de Escherichia
coli.
Se realizaron 18 capacitaciones de BPM
durante el año y del cual una capacitación de 2 horas fue
requerida por dueño, manipulador/a y/ó dueño; de ello
fueron capacitados 409 personas. <=
o:p>
Así como para su anális=
is
se aplicó el programa de Excel para la Interpretación de
resultados.
Resultados
De las 455
muestras analizadas que tuvieron Es=
cherichia
coli como indicador de contaminación fecal, se llevó a ca=
bo
un segundo muestreo para determinarles los microorganismos patógenos,
según la materia prima entregada en cada expendio, indicando que fue=
ron
215 alimentos y se realizó de un mismo alimento, ente 1 y 3
patógenos más frecuentes que se concentran en dicha materia
prima; para lo cual fue un total de 600 análisis microbiológi=
cos.
En la tabla 1 =
se
presentan los alimentos muestreados según tiempo de comida y/o
descripción específica de la muestra procesada; siendo:<=
/o:p>
Tabla No. 1
Mues=
tras
de Alimentos obtenidas en establecimientos de alimentos
Se
pudo observar que las refacciones, vegetales y carnes son las más
consumidas por los consumidores y por ende es donde se requiere que se apli=
quen
mejor las Buenas Prácticas de Manufactura.
A
continuación, se presenta la figura 1 donde se muestra
gráficamente las muestras de alimentos obtenidas en los establecimie=
ntos
de alimentos y donde los productos de refacciones, vegetales, carnes y
lácteos son los que más se expenden en dichos establecimiento=
s.
=
Figura
1.
=
Cantidad y Muestras de Alimentos obtenidas en
establecimientos de alimentos
Se
pudo observar que las refacciones, vegetales, bebidas, carnes y láct=
eos
son las más consumidas por los consumidores y por ende es donde se
requiere aplicar las Buenas Prácticas de Manufactura.
A
continuación, se encuentra la tabla 2, donde se presentan los
microorganismos aislados de las diversas muestras procesadas.
Tabla 2.
Microorganismos m&aacut=
e;s
frecuentemente aislados en alimentos obtenidos de Establecimientos de alime=
ntos
Microrganismo
Muestras
Rango
de aceptabilidad
(Según
Parámetros del
Laboratorio)
Cantidad
de Muestras del rango de aceptabilidad
Cantidad
de Muestras fuera del rango de aceptabilidad
Salmonella s pp
79
Ausencia
79
---
Shigella s pp
71
Ausencia
71
---
Grupo Coliforme
85
Hasta
1,000 UFC/g
73
12
E. coli
85
Hasta
10 UFC/g ó ml
1
84
Mohos y levaduras
114
Hasta
1,000 UFC/g
4
110
Recuento Aeróbico Total=
104
Hasta
1,000 UFC/g
26
78
Staphylococcus aureus
62
Hasta
1,000 UFC/g
57
5
TOTAL
600
311
289
Como
se puede observar el laboratorio debe cumplir con las normativas del Minist=
erio
de Salud, donde se indica que, si hay indicador de contaminación ora=
l –
fecal (Escherichia coli ), debe realizarse el análisis de
patógenos para la respectiva detección de una intoxicaci&oacu=
te;n
e infección alimentaria y que forma parte de las enfermedades
transmitidas por alimentos - ETA’s –; por ello se presenta la
cantidad de muestras que están dentro y fuera del rango de
aceptabilidad.
Asimismo,
se presenta una gráfica donde se demuestra la aceptabilidad de
microorganismos más frecuentemente aislados en los alimentos muestre=
ados
y que se demuestra que está dentro del rango de aceptabilidad del
laboratorio.
Figura 2.
Microorganismos má=
;s
frecuentemente aislados en alimentos y que se encuentran dentro del Rango de
Aceptabilidad
Por
otro lado, se presenta la gráfica que demuestra la cantidad de muestras que se
encuentran fuera del rango de aceptabilidad de microorganismos
más frecuentemente aislados en los alimentos muestreados y que
están basados en el r ango
de aceptabilidad de los parámetros del laboratorio.
Figura 3.
Microorganismos má=
;s
frecuentemente aislados en alimentos y que se encuentran fuera del Rango de
Aceptabilidad
<=
span
style=3D'mso-fareast-language:ES-EC;mso-no-proof:yes'>
Como se puede
demostrar que el patógeno Staphylococcus aureus present&oacut=
e; 5
materias primas que estuvieron fuera del rango de aceptabilidad, lo que ind=
ica
que puede llegar a producir una intoxicación alimentaria, ya que
está fuera del rango de aceptabilidad que cuenta el laboratorio.
Asimismo se in=
dica
que en base a los alimentos analizados el microorganismo que más se
encontró fue
Grupo Coliforme, E. coli, como los <=
/b>patógenos Salmonella sp p y Shigella sp p; los cuales se
realizan para demostrar si dentro de los indicadores procesados, existe la
presencia de patógenos productores de infecciones alimentarias y con
ello determinar si cumplen con las BPM; de estos resultados el 100% de Salmonella sp p y Shigella sp p se encuentran =
dentro
de los límites de aceptabilidad; pero se puede determinar que la
aplicación de BPM para la desinfección adecuada de materia pr=
ima
en esas materias primas si se cumple; pero en otras muestras procesadas se
encontró que el 16.44 % se encontró los microorganismos del G=
rupo
coliforme y asimismo se encontró que el 98.82 % se aisló E.coli , lo que indica que se obtuvieron resultados indicativos=
de
una contaminación fecal de origen humano, indicando que 84 de 85
alimentos analizados fueron no aptos para consumo humano y por ello se indi=
ca
que en los establecimientos donde se obtuvieron esas muestras no cumplen con
las buenas prácticas de manufactura al momento de la elaboraci&oacut=
e;n
de los alimentos.
Por otro lado =
se
indica que otro de los indicadores de contaminación que se realizan =
para
determinar si la manipulación, conservación y almacenamiento es correcto es la
prueba de Recuento aeróbico total;&=
nbsp;
mohos y levaduras y de los cuales se realizaron 114 y 104 procesos
respectivamente; de los cuales se obtuvo que el 96.49 % de muestras no cump=
len
con las BPM durante el proces=
o de
vida de anaquel de mohos y levaduras; también se indica que el 75% de
muestras no cumplen con la desinfección adecuada de preparació=
;n y
selección de materia prima de los establecimientos muestreados, basa=
do
en el recuento aeróbico total.
De los resulta=
dos
para demostrar la presencia de una intoxicación alimentaria, la prue=
ba
es la determinación de Staphylococcus aureus , la cual se realizaron 62
análisis y se obtuvo que 5 (8.77 %) alimentos, sobrepasaron el
límite de aceptación y del cual se determina que pudo existir=
una
intoxicación alimentaria en más de algún alimento
preparado.
Por último se indica que fueron convocad=
os a
recibir una capacitación y se llevaron a cabo 18 formaciones durante
todo el año con 2 horas de duración por persona; de ello se
indica que asistieron 409 personas y/o dueños y/o manipuladores; de =
los
cuales el 40% fue del sexo masculino y el 60% del sexo femenino; en dicha c=
apacitación
se les formó desde la selección, compra, almacenamiento y pro=
ceso
a la hora de preparar los alimentos; así como cuales son las consecuencias de no
cumplir con las buenas prácticas de manufactura.
Conclusiones
·=
; =
De los resultados obtenidos se puede
concluir que cada población universitaria debe implementar mensualme=
nte
un control a los establecimientos de alimentos que expenden alimentos, para
detectar a tiempo las posibles enfermedades transmitidas por alimentos
-ETA´s- y con ello realizar un análisis minucioso de las afecc=
iones
recurrentes que existen en los diversos establecimientos de alimentos. Por =
otro
lado, es importante indicar que se debe realizar en una próxima
investigación una correlación de los resultados obtenidos del
control realizado con las fuentes de contaminación como agua, lavado=
de
manos y evaluación de la verificación de la desinfecció=
;n
de superficies en los diversos establecimientos que tiene la Universidad
Pública de Guatemala.
·=
; =
La ausencia de patógenos como <=
i>Salmonella
spp y Shigella sp p en esta investigación, ya que son
indicativos frecuentes de enfermedades transmitidas de alimentos-ETA’=
s-,
indica que en los establecimientos investigados se cumple con la normativa
obligatoria del Ministerio de Salud Pública de Guatemala y que siguen
con las normas sanitarias de limpieza, higiene e inocuidad de Alimentos.
·=
; =
Por otro lado se indica que el 98.82 %=
del
hallazgo de E.coli en los 455
alimentos, obtenido de los 182 establecimientos y que corresponden al indic=
ador
de contaminación fecal de origen humano; su presencia es indicativa =
que
las BPM no son bien aplicadas por los manipuladores de expender alimentos y=
por
ello se concluye que no están expendiendo alimentos aptos para consu=
mo
humano y que además es urgente realizar acciones de mejora, o bien
revisar el Rango de aceptabilidad de los parámetros que tiene el
laboratorio y por ello se recomienda realizar una correlación con los
resultados de las muestras coprológicas y de coprocultivo de los
manipuladores de los establecimientos; así también determinar=
le
el análisis de lavado de manos para determinar la interrelació=
;n y
procedencia de la contaminación fecal de origen humano.
·=
; =
En relación con los resultados =
de Staphylococcus
aureus que 5 de 62 análisis ( 8.77 % ) alimentos,
sobrepasaron el límite de aceptación que tiene el laboratorio=
hasta 1,000 UFC/g, y en cambio en el
Reglamento Técnico Centroamericano (22) – RTCA
67.04.50:08, en diversas materias primas va del rango de < 10 hasta 1,000
UFC/g; lo que implica que aunque el porcentaje no es alto, pero pudo
presentarse más de alguna una intoxicación alimentaria en
algún consumidor de algún establecimiento y por ello se
recomienda difundir ante la población consumidora los síntoma=
s y
signos que conlleva consumir alimentos intoxicados y con ello al laboratori=
o se
le recomienda implementar estrategias de seguimiento inmediatas como manten=
er
la correlación de los resultados microbiológicos del lavado de
manos, control de salud y
supervisión para lograr la inocuidad alimentaria. =
·=
; =
Respecto al hallazgo de Mohos y Levadu=
ras
con 96.49 % (110 de 114) de
alimentos analizados, se concluyó que no se está cumpliendo c=
on
las práctica de vida de anaquel y que por ello es importante indicar=
y
reforzar en la capacitación de manipuladores que la materia prima qu=
e se
adquiere debe tener una ampliación de fecha de vencimiento; ó
también se podría ampliar el rango de aceptabilidad por parte=
del
laboratorio, ya que en el Reglamento Técnico Centroamericano –
RTCA 67.04.50:08, únicamente se requiere para Jaleas, mermeladas y
rellenos de frutas para repostería.
·=
; =
De igual forma para el análisis=
de
Recuento Aeróbico Total se encontró que el 75 % (78 de 104) de
alimentos analizados no cumplen con las buenas prácticas de manufact=
ura
respecto a las condiciones del manejo de control de temperatura; que indica=
que
no la manipulan adecuadamente, desde su limpieza, desinfección,
cocimiento hasta el almacenamiento conveniente después de su respect=
iva
elaboración; pero también&nb=
sp;
se indica que basados en el
rango de aceptabilidad por parte del laboratorio es muy amplio ( Hasta 1,000 UFC/g)
comparado con el Reglamento Técnico Centroamericano – RTCA (22=
),
67.04.50:08, que se requiere que sea un rango < 10 UFC/g ó ml; po=
r lo
que se sugiere implementar controles de seguimiento de las BPM y que se asi=
gne
a un manipulador responsable de cada establecimiento para llevar el registro
respectivo desde la compra, limpieza, desinfección y almacenamiento =
previo
a su preparación final.
·=
; =
Por último, se concluye que se
deben implementar las sugerencias derivadas de los resultados de este
artículo para que se mantengan los límites de inocuidad
alimentaria y además se sugiere que los manipuladores de alimentos d=
eben
ser capacitados como mínimo dos veces al año, con el fin de q=
ue
las buenas prácticas de manufactura logren incrementar la higiene e
inocuidad alimentaria.
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&nb=
sp;
El artículo que se publica es de exclusiva
responsabilidad de los autores y no necesariamente reflejan el pensamiento =
de
la Revista Anatomía Digital=
.
&nb=
sp;
El
artículo queda en propiedad de la revista y, por tanto, su
publicación parcial y/o total en otro medio tiene que ser autorizado=
por
el director de la Revista Anatomía Digital . =
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